Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus

Figure 7

Determination of the thermostability of Tth pol III HE in cycled reactions. (a) The effect of cycle number on the amount of DNA synthesized. (b) Products from the asymmetric PCR-like reactions. The circular ssM13mp18 DNA served as the starting material. 0 cycles refers to the mix that was not cycled at any temperature. All other lanes contain products from reactions cycled for the indicated times. Each cycle consisted of steps at 94°C/30 s, 60°C/1 min, and 70°C/2 min. The noncycled reaction contains primed M13mp18 template plus Tth pol III HE incubated at 70°C for 2 min.

(a)

(b)