Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus

Figure 8

PCR reactions for amplification of short regions of DNA. PCR reactions and agarose gel analysis were as described under Section 2. PCR cycles consisted of 94°C/30 s, 55°C/1 min, and 72°C/2 min. Taq Pol indicates PCR reactions using Taq DNA polymerase (18038-018, Invitrogen) per manufacturer’s instructions. Primers used are shown in Table 3. (a) PCR reactions using primers designed to yield a 200 bp PCR product. (b) PCR reactions using primers designed to yield a 512 bp PCR product. The number of PCR cycles is indicated at bottom of the figures.

(a)

(b)