Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus

Figure 9

PCR reactions to amplify intermediate and long regions of DNA. Reactions were conducted as described under Section 2. PCR reactions were allowed to proceed for 30 cycles. The same amount of primer and template was used in Tth pol III HE and Taq polymerase reactions. Primers used are shown in Table 3. PCR product sizes are as indicated. (a) The template was pET Blue-2 plasmid (Novagen) and primers were designed to yield 1500 and 2500 bp products. (b) The template was Lambda DNA-HindIII Digest and primers were designed to yield a 4650 and 7500 bp product. (c) The template was Lambda DNA-HindIII Digest and primers were designed to yield a 12500 and 15000 bp product.

(a)

(b)

(c)