Research Article
Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus
Table 1
Primers used for PCR amplification of genes encoding Tth pol III holoenzyme subunits.
| Gene amplified | Primer direction | Sequence (5′-3′) |
| dnaE5′ | Sense | GGATATGCATTGAGGAGGATCGATTAATGGGCCGCAAACTCCGC | dnaE5′ | Antisense | CGGCTCGCCAGGCGCACCAGG | dnaN | Sense | ACTGATCGATTAATGAACATAACGGTTCCCAAG | dnaN | Antisense | GACTACTAGTCTACTAGACCCTGAGGGGCACCACC | dnaX | Sense | ACTGATCGATTAATGAGCGCCCTCTACCGCCGC | dnaX | Antisense | GACTACTAGTTTATTATATACCAGTACCCCCTATC | holA | Sense | ACTGATCGATTAATGGTCATCGCCTTCACCGGGG | holA | Antisense | GACTACTAGTCATCAACGGGCGAGGCGGAGG | holB | Sense | ACTGATCGATTAATGGCTCTACACCCGGCTCACCC | holB | Antisense | GACTACTAGTCATCATGTCTCTAAGTCTAAGGCC | ssb | Sense | ACTGATCGATTAATGGCTCGAGGCCTGAACCGCG | ssb | Antisense | GACTACTAGTCATCAAAACGGCAAATCCTCCTCC |
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