Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus

Table 1

Primers used for PCR amplification of genes encoding Tth pol III holoenzyme subunits.

Gene amplified	Primer direction	Sequence (5′-3′)
dnaE5′	Sense	GGATATGCATTGAGGAGGATCGATTAATGGGCCGCAAACTCCGC
dnaE5′	Antisense	CGGCTCGCCAGGCGCACCAGG
dnaN	Sense	ACTGATCGATTAATGAACATAACGGTTCCCAAG
dnaN	Antisense	GACTACTAGTCTACTAGACCCTGAGGGGCACCACC
dnaX	Sense	ACTGATCGATTAATGAGCGCCCTCTACCGCCGC
dnaX	Antisense	GACTACTAGTTTATTATATACCAGTACCCCCTATC
holA	Sense	ACTGATCGATTAATGGTCATCGCCTTCACCGGGG
holA	Antisense	GACTACTAGTCATCAACGGGCGAGGCGGAGG
holB	Sense	ACTGATCGATTAATGGCTCTACACCCGGCTCACCC
holB	Antisense	GACTACTAGTCATCATGTCTCTAAGTCTAAGGCC
ssb	Sense	ACTGATCGATTAATGGCTCGAGGCCTGAACCGCG
ssb	Antisense	GACTACTAGTCATCAAAACGGCAAATCCTCCTCC