High Diversity in Iron Cycling Microbial Communities in Acidic, Iron-Rich Water of the Pyhäsalmi Mine, Finland
Table 1
The control strains used in the PCR tests for the genus-specific primers. All primers were tested on the same DNA preparates, i.e., the amount of DNA used in the universal bacterial and Acidithiobacillus sp. 16S rRNA gene targeting reactions is the same. However, DNA concentration may vary between preparates of different species.
Strain
Taxonomy (class; order)
Ct values
P1/P2
Acidithiobacillus
Acidithiobacillus ferrooxidans
Acidithiobacilli; Acidithiobacillales
18.87
13.46
Methylosinus trichosporium
Alphaproteobacteria; Rhizobiales
15.16
21.87
Alcaligenes faecalis
Betaproteobacteria; Burkholderiales
20.26
30.57
Leptothrix discophora
Betaproteobacteria; Burkholderiales
15.79
9.81
Desulfovibrio desulfuricans
Deltaproteobacteria; Desulfovibrionales
20.53
25.27
Escherichia coli
Gammaproteobacteria; Enterobacteriales
20.10
23.66
Pseudomonas putida
Gammaproteobacteria; Pseudomonadales
10.58
7.14
Alcanivorax borkumensis
Gammaproteobacteria; Oceanospirillales
12.38
15.25
Leptospirillum ferrooxidans
Nitrospira; Nitrospirales
22.84
—
Sulfobacillus benefaciens DSM 19468
Bacilli; Bacillales
19.06
32.66
Clostridium ljungdahlii E-153486
Clostridia; Clostridiales
18.70
32.55
Mock community DNA#
7.21
17.51
Methanothermobacter thermautotrophicum DSM 1053
Methanobacteria; Methanobacteriales
17.58
—
Sulfolobus solfataricus DSM 1616
Thermoprotei; Sulfolobales
26.81
—
Halobacterium salinarum
Halobacteria; Halobacteriales
27.42
—
Archaea; #mock community DNA (ZymoBIOMICS™ Microbial Community Standard, Zymo Research Inc., Irvine, CA, USA); − indicates that no amplification was detected.
