Research Article

High Diversity in Iron Cycling Microbial Communities in Acidic, Iron-Rich Water of the Pyhäsalmi Mine, Finland

Table 3

The number of sequences, OTUs, Chao1 estimated number of OTUs, Shannon’s diversity index, and number of identified genera of the bacteria, fungi, and archaea found in the original mine water samples and enrichments. The values in grey indicate that only low numbers of sequence reads were obtained and the data was excluded from the analyses. The sample name abbreviations are as follows; P = Pyhäsalmi; 240 m, 500 m, 600 m = depth; A, B = sample replicate A or B; 176 = ferrous iron-containing medium 176; 180 = ferrous iron-containing medium 180; Fe(III) = ferric iron containing growth medium; Ye = Yeast extract containing growth medium.

SamplesNumber of sequencesNumber of OTUsEstimated number of OTUs (Chao1)Shannon’s diversity indexNumber of genera

Bacteria
P240m_A16533094754,863
P240m_B22793245204,670
P240m_176
P240m18029863425384,090
P240m_Fe(III)7872615144,675
P240m_Fe(III)Ye22503075194,084
P500m_A900359910704,0104
P500m_B50984688774,180
P500m_17664523425302,877
P500m18022913034824,078
P500m_Fe(III)5521482833,450
P500m_Fe(III)Ye13752734923,878
P600m_A18423396604,370
P600m_B600287914875,3114
P600m_17647232964602,784
P600m18022593376034,578
P600m_Fe(III)27953035143,072
P600m_Fe(III)Ye19873345384,489

Fungi
P240m_A2217473
P240m_B435576818665104
P240m_176
P240m1806113164133676111
P240m_Fe(III)5764231941467120
P240m_Fe(III)Ye187888923526103
P500m_A3712156631297114
P500m_B4174169035517117
P500m_1763049127030017114
P500m1803868150831707110
P500m_Fe(III)219989028136109
P500m_Fe(III)Ye4978141027356117
P600m_A100825204
P600m_B7544187137916114
P600m_1763040122728376111
P600m1803666110021636109
P600m_Fe(III)3537137928887104
P600m_Fe(III)Ye327199125136110

Archaea
P240m_A2231
P240m_B1810132
P240m_176
P240m18011331
P240m_Fe(III)
P240m_Fe(III)Ye3611142
P500m_A8001376535410
P500m_B7228347586410
P500m_17620434762
P500m1801110
P500m_Fe(III)
P500m_Fe(III)Ye
P600m_A2814322
P600m_B4499206379313
P600m_1763035141308210
P600m18044101