Frequencies of CYP2B6^∗4,^∗5, and ^∗6 Alleles within an Iranian Population (Mazandaran)

Table 1

Specific primers for amplification and evaluation of each CYP2B6 defective allele.

CYP2B6 allele	Specific primer pairs	Annealing Tm	PCR product sizes
^∗4	F: 5′GACAGAAGGATGAGGGAGGAA3′	59°C	640 bp
	R: 5′CTCCCTCTGTCTTTCATTCTGT3′
^∗5	F: 5′ACAAGAATCATTTGAACCACCTG3′	59°C	600 bp
	R: 5′AGTCAGAGCCATTGTCTACAG3′
^∗6	F: 5′TCTCGGTCTGCCCATCTATAAACT3′	59°C	401 bp
	R: 5′CCTGACCTGGCCGAATACA3′