Research Article

Effects of Genetic Variants in the Nicotine Metabolism Pathway on Smoking Cessation

Table 3

Primers used for amplification of CYP2A64 (gene deletion).

ReactionPrimer namePrimer sequenceProduct size (bp)

PCR I2Aex7F5′-CCAAGATGCCCTACATG-3′1,967
2A6R15′-CTTATGTTTTGTGAGACATCAGAGACAA-3′

PCR II2A6ex8F5′-CACTTCCTGACTGAG-3′1,180
2A7ex8F5′-CATTTCCTGGATGAC-3′
2A6R25′-AAAATGGGCATGAACGCCC-3′

F forward primer, R reverse primer.