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Genetics Research
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2022
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Article
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Tab 3
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Research Article
Effects of Genetic Variants in the Nicotine Metabolism Pathway on Smoking Cessation
Table 3
Primers used for amplification of
CYP2A6
4
(gene deletion).
Reaction
Primer name
Primer sequence
Product size (bp)
PCR I
2Aex7F
5′-CCAAGATGCCCTACATG-3′
1,967
2A6R1
5′-CTTATGTTTTGTGAGACATCAGAGACAA-3′
PCR II
2A6ex8F
5′-CACTTCCTGACTGAG-3′
1,180
2A7ex8F
5′-CATTTCCTGGATGAC-3′
2A6R2
5′-AAAATGGGCATGAACGCCC-3′
F
forward primer,
R
reverse primer.