Triptolide Inhibits Th17 Response by Upregulating microRNA-204-5p and Suppressing STAT3 Phosphorylation in Psoriasis

Figure 1

The effects of triptolide on cell viability and IL-17 differentiation. (a) Triptolide chemical structure. (b) MTT assay conducted to detect the viability of splenocytes which were isolated from mice in the presence or absence of increasing dosages of triptolide (1–500 nM) treatment. (c) Anti-CD3 or anti-CD3 + IL-6 + TGF-β used to stimulate the isolated splenocytes from mouse models with or without 1.25–10 nM triptolide treatment. ELISA was used for measuring IL-17 concentration in the supernatants. (d) IL-17⁺CD4⁺ T population determined by flow cytometry analysis. Each bar presents the mean ± standard deviation obtained from three to four separate experiments. , compared to the model group. n.s indicates no significance.

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