Improved Recovery of Exfoliated Colonocytes from Feces Using Newly Developed Immunomagnetic Beads
Figure 1
Simulation to evaluate the recovery rates of colonocytes using several sizes of beads. (a) Electron microscopy images show 3 different sizes of magnetic beads prepared by us. The sizes are 3.0, 4.9, and 5.9 μm. Scale bar, 50 μm. (b) Comparison of cell recovery rates. The cell recovery rates using beads A (smallest, 3.0 μm), beads B (medium, 4.9 μm), and beads C (largest, 5.9 μm) are 65.9 ± 1.37 (%, mean ± SD), 61.1 ± 0.98, and 57.1 ± 0.75, respectively. The columns show the cell retrieval rates and the bars show the standard deviations. Significant differences are showed by an asterisk (*, P = .0001) or a double asterisk (**, P < .0001). (c) Papanicolaou staining of HT-29 cells captured using immunomagnetic beads. The arrowheads show the cell-bead complexes. The beads A have adhered to the HT-29 cells with the highest densely. Scale bar, 50 μm.
