Improved Recovery of Exfoliated Colonocytes from Feces Using Newly Developed Immunomagnetic Beads
Figure 3
Cell retrieval ability of newly developed immunomagnetic beads in clinical samples. (a) Cell retrieval ability using several sized immunomagnetic beads in clinical samples. The median amounts of DNA from 2-gram stool using beads A (smallest, 3.0 μm), beads B (medium, 4.9 μm), and beads C (largest, 5.9 μm) are 1.50 ng (range; 0.09–8.12), 0.58 ng (range; 0–4.23), and 0.25 ng (range; 0–4.62), respectively. The horizontal bar is a median amount of extracted DNA from isolated colonocytes from 2-gram stool; the upper vertical bar is a maximal amount of extracted DNA; the lower vertical bar is a minimal amount of extracted DNA; and the column contained 50% of these populations. (b) Cell retrieval ability using immunomagnetic beads tagged with several affinities mAbs in clinical samples. The median amounts of DNA from 2-gram stool using beads D (tagged with a lower affinity mAb, Clone 1-2), beads E (tagged with a higher affinity mAb, Clone B8-4), and beads F (tagged with a higher affinity mAb, Clone B8-7) are 0.75 ng (range; 0.03–3.65), 1.66 ng (range; 0.12–5.74), and 0.99 ng (range; 0.08–5.67), respectively. The horizontal bar is a median amount of extracted DNA from isolated colonocytes from 2-gram stool; the upper vertical bar is a maximal amount of extracted DNA; the lower vertical bar is a minimal amount of extracted DNA; and the column contained 50% of these populations.
