Differences in Protein Expression and Gene Amplification of Cyclins between Colon and Rectal Adenocarcinomas

Table 1

Primary antibodies used for immunohistochemistry.

Antibody	Retrieval method	Dilution	Incubat time	Host species	Clone	Vendor
Cyclin A2	Tris/EDTA, pH 9	1∶150	32 min	Mouse, monoclonal	6E6	Novocastra*
Cyclin B1	Tris/EDTA, pH 9	1∶40	30 min	Mouse, monoclonal	7A9	Novocastra*
Cyclin D1	Dako TRS, pH 6	1∶50	30 min	Rabbit, monoclonal	SP4	Lab Vision^†
Cyclin D3	Tris/EDTA, pH9	1∶20	30 min	Mouse, monoclonal	DCS-22	Novocastra*
Cyclin E	Tris/EDTA, pH9	1∶40	30 min	Mouse, monoclonal	13A3	Novocastra*
c-Myc	Tris/EDTA, pH 9	1∶50	30 min	Mouse, monoclonal	9E11	Novocastra*
𝛽-catenin	Tris/EDTA, pH 9	1∶300	30 min	Mouse, monoclonal	17C2	Novocastra*

∗Novocastra, Newcastle, UK
†Lab Vision, Fremont, CA.