Differences in Protein Expression and Gene Amplification of Cyclins between Colon and Rectal Adenocarcinomas

Table 2

Primer and probe sequences for cyclins used in real time quantitative polymerase chain reaction.

Gene	Primer sequence (5^′-3^′)	Hybridisation probe sequence (5^′-3^′)
CCNA2
Forward	GCCACAGTAGGAGTTCTCCCATAT	FAM-CCCCGCCAACACTG-NFQ
Reverse	CAGACCGGCAGCATACACA
CCNB1
Forward	CCCTGCTGCAACCTCCAA	FAM-CCCGGACTGAGGCCAAGAACAGC-TAMRA
Reverse	TGTTCACTGACTTTGTTACCAATGTC
CCND1
Forward	CCGTCCATGCGGAAGATC	FAM-CCTCCAGCATCCAGGTGGCGA-TAMRA
Reverse	AACAAGTTGCAGGGAAGTCTTAAGA
CCND3
Forward	CTGTCTCTCCCCGCCAGTT	FAM-CACCCCCGACACGTATTGTCTCCC-TAMRA
Reverse	CTGATATCTCAAGCTTTCCTTTTCCT
CCNE
Forward	CCCCGCTGCCTGTACTGA	FAM-TCAGTGCCGACTCTGCCACATGG-TAMRA
Reverse	AGCATGGAGTAAGAGACCTGGAA