Differences in Protein Expression and Gene Amplification of Cyclins between Colon and Rectal Adenocarcinomas

Table 6

Amplification of cyclin genes of the rectal cancers.

Gene	𝑁 (total)	Amplification level*
		<0.5	0.5–1.9	2.0–4.9	≥5
Cyclin A2	237	9	206	21	1
Cyclin B1	236	4	229	3	0
Cyclin D1	236	5	212	19	0
Cyclin D3	234	22	207	5	0
Cyclin E	235	2	191	40	2

∗N-fold difference from the normal controls. Amplification was measured by real-time quantitative polymerase chain reaction, and levels were determined by the comparative C_t method (2^−ΔΔCt method).