Rat Strain Differences in Susceptibility to Alcohol-Induced Chronic Liver Injury and Hepatic Insulin Resistance
Figure 6
Effects of strain chronic ethanol exposure on insulin, IGF-1, and IGF-2 receptor binding: competitive saturation binding assays were used to measure specific insulin, IGF-1, and IGF-2 receptor binding with [125I] labeled insulin, IGF-I, or IGF-2 in the presence or absence of cold ligand. Radiolabeled bound ligand was harvested onto GF/C filter plates and measured in a TopCount. Specific binding curves (mean S.D.) are depicted for (a)–(f) insulin, (g)–(l) IGF-I, or (m)–(r) IGF-2 receptors in FS (a, g, m), LE (b, h, n), and SD (c, i, o) controls or FS (d, j, p), LE (e, k, q), and SD (f, l, r) ethanol-exposed livers. Statistics are shown in Table 3.
