Gastroenterology Research and Practice

Research Article

Rat Strain Differences in Susceptibility to Alcohol-Induced Chronic Liver Injury and Hepatic Insulin Resistance

Table 1

Primer pairs for quantitative RT-PCR assays.


Primer		Sequence ()	Position	Size (bp)

Desmin	For	ACCTGCGAGATTGATGCTCT	1069	206
Desmin	Rev	ACATCCAAGGCCATCTTCAC	1274
COL1a2	For	ACCTCAGGGTGTTCAAGGTG	1632	222
COL1a2	Rev	CGGATTCCAATAGGACCAGA	1844
-SMA	For	TGTGCTGGACTCTGGAGATG	516	148
-SMA	Rev	GAAGGAATAGCCACGCTCAG	663
ASBT	For	GCATTGGCATTTCTCTGGTT	598	181
ASBT	Rev	GGTTCAATGATCCAGGCACT	778
GFAP	For	GGTGGAGAGGGACAATCTCA	433	215
GFAP	Rev	CTCGAACTTCCTCCTCATGG	647
ALB	For	CTTCAAAGCCTGGGCAGTAG	702	188
ALB	Rev	TGGAGATAGTGGCCTGGTTC	889
KCR	For	TCACAAATGCTGTGGACCAT	1427	161
KCR	Rev	GTCTTCACGCTCTCCGTTTC	1587
ADH-1	For	TGCTCCGTGCTGGAAAGAGTATCC	1138	117
ADH-1	Rev	TAAGGTTGTGATGTGGCTGGCG	1254
ADH-7	For	CAATGCTGCTTTTCACTGGA	971	234
ADH-7	Rev	AGAACACCCAGCTCTCTGGA	1204
ALDH-1	For	ATCTGCCATGTGGAAGAAGG	173	216
ALDH-1	Rev	CAAGTACGCATTGGCAAAGA	388
ALDH-2	For	GACCTGGACAAGGCCAATTA	1391	193
ALDH-2	Rev	TCTTCTGTGGCACTTTGACG	1583
ALDH-3	For	CTGATTGCTGAGGTTCCTGTTAGG	1914	119
ALDH-3	Rev	GGATGTTTAGACTGAGAGCCGACTC	2032
Catalase	For	ATACGAAGGTGTTGAATGAGGAGG	1369	63
Catalase	Rev	TCAGGTGGTTGGCAATGTTCTC	1431
CYP2E1	For	GTGTTCACACTGCACCTTGG	211	69
CYP2E1	Rev	CACCTCCTTGACAGCCTTGT	279
GAPDH	For	AGT GGG CAT CAA TGG ATT TGG	306	241
GAPDH	Rev	GGG GAT TTC CTT AGG TTC TTT GC	546
IL-1	For	CAG CAG CAT CTC GAC AAG AG	233	60
IL-1	Rev	CTT CTC CAC AGC CAC AAT GA	292
TNF-	For	ATG TGG AAC TGG CAG AGG AG	26	84
TNF-	Rev	AGA AGA GGC TGA GGC ACA GA	109
IL-6	For	ATG TTG TTG ACA GCC ACT GC	116	51
IL-6	Rev	GTC TCC TCTCCG GAC TTG TG	166

qPCR: quantitative polymerase chain reaction; ASBT: apical sodium dependent bile acid transporter; KCR: Kupffer cell glycoprotein receptor; GFAP: glial fibrillary acidic protein; COL1a2: pro-alpha-2(I) collagen; ALB: albumin; -SMA: alpha smooth muscle actin; ADH: alcohol dehydrogenase; ALDH: aldehyde dehydrogenase; CYP2E1: cytochrome P450 2E1; GAPDH: glyceraldehyde-3-phosphaye dehydrogenase; IL: interleukin 1; TNF: tumor necrosis factor.