The Prognostic Impact of Protein Expression of E-Cadherin-Catenin Complexes Differs between Rectal and Colon Carcinoma

Table 2

Primary antibodies used for immunohistochemistry.

Antibody	Retrieval method	Dilution	Incubat time	Host species	Clone	Vendor
𝛼-catenin	Dako TRS high pH	1 : 150	30 minutes	Mouse, monoclonal	25B1	BD Biosciences Europe*
𝛽-catenin	Tris/EDTA pH 9	1 : 300	30 minutes	Mouse, monoclonal	17C2	Novocastra^†
𝛾-catenin	Tris/EDTA	1 : 25	30 minutes	Mouse, monoclonal	11B6	Novocastra^†
p120-catenin	Dako TRS high pH	1 : 75	30 minutes	Mouse, monoclonal	15D2	Santa Cruz Biotechnology^‡
E-cadherin	Dako TRS high pH	1 : 100	30 minutes	Mouse, monoclonal	NCH-384	Dako**

^∗BD Bio Sciences Europe, [email protected].
^†Novocastra, Newcastle, UK.
^‡Santa Cruz Biotechnology, Inc., [email protected].
^∗∗Dako, N-1086 Oslo, Norway.