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Gastroenterology Research and Practice
Volume 2010 (2010), Article ID 640797, 14 pages
http://dx.doi.org/10.1155/2010/640797
Research Article

Rosuvastatin Counteracts Vessel Arterialisation and Sinusoid Capillarisation, Reduces Tumour Growth, and Prolongs Survival in Murine Hepatocellular Carcinoma

1Institut des Vaisseaux et du Sang, Hôpitlal Lariboisière, 2 Rue Ambroise Paré, 75010 Paris, France
2“Angiogenèse et Recherche Translationnelle” INSERM U 965 “Equipe labellisée Ligue 2009”, HÔpital Lariboisière, 2 Rue Ambroise Paré, 75010 Paris, France
3Université Paris 7 Denis Diderot, 10 Avenue de Verdun, 75010 Paris, France
4Laboratoire M.E.R.C.I, Université de Rouen, 22 Boulevard Gambetta, 76183 Rouen Cedx, France
5HÔpital Lariboisière, 2 Rue Ambroise Paré, 75010 Paris, France
6Paris Cardiovascular Research Center, INSERM U970, HÔpital Européen Georges Pompidou, 20 Rue Leblanc, 75015 Paris, France
7HÔpital Européen Georges Pompidou, 20 Rue Leblanc, 75015 Paris, France
8Université Descartes-Paris 5, 12 Rue de l’École de Médecine, 75006 Paris, France

Received 29 September 2010; Accepted 28 December 2010

Academic Editor: Fabio Marra

Copyright © 2010 Annemilaï Tijeras-Raballand et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In normal livers, desmin‐stained mural cells in vessels derived from the portal tract and SMAstained smooth muscle cells in arteries (Supplementary Figure 1(a, b). CD31‐positive sinusoidal ECs were surrounded by non‐activated HSCs expressing desmin in normal, untreated and rosuvastatin‐treated HCC livers ((Supplementary Figure 1(a)) whilst they were surrounded by activated HSCs expressing SMA only in HCC livers (Supplementary Figure 1(b)). Interestingly, in rosuvastatin‐treated HCC livers, CD31‐positive sinusoidal ECs were more intensely surrounded by non‐activated desmin‐positive HSCs than in untreated HCC livers (Supplementary Figure. 1(a)).

Representative images of negative controls. The primary antibody was omitted, or incubated with an excess of blocking peptide as a negative control (Supplementary Figure. 2).

  1. Supplementary Figures