The Regenerating Gene I𝜶 Is Overexpressed in Atrophic Gastritis Rats with Hypergastrinemia

Figure 3

The RegIα protein and its receptor mRNA were overexpressed in atrophic gastritis rats with hypergastrinemia. (a) The average serum gastrin levels (ng/mL) in atrophic gastritis and normal rats were determined by ELISA assay. (b) Representative results of RegIα protein expression in gastric tissues from atrophic gastritis and normal rats (upper lane) were performed by immunoblot experiments. Band densities were quantified and protein levels were normalized to β-actin (lower diagram). (c) The expression of RegIα receptor mRNA was measured by quantity real-time PCR and calculated using the value of 2^−ΔΔCT. Relative fold change in atrophic gastritis group was compared with that of normal group, which was normalized as a reference value of 1.0. Control group: normal control rats; Model group: atrophic gastritis rats. *𝑃<0.05, **𝑃<0.01.