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Gastroenterology Research and Practice
Volume 2011 (2011), Article ID 578691, 8 pages
http://dx.doi.org/10.1155/2011/578691
Research Article

The Effect of Antivascular Endothelial Growth Factor on the Development of Adhesion Formation in Laparotomized Rats: Experimental Study

1Department of General Surgery, Diyarbakir Education and Research Hospital, 21400 Diyarbakir, Turkey
2Department of General Surgery, Faculty of Medicine, Firat University, 23100 Elazig, Turkey
3Department of Medical Pathology, Faculty of Medicine, Firat University, 23100 Elazig, Turkey

Received 16 February 2011; Accepted 2 May 2011

Academic Editor: Hirozumi Sawai

Copyright © 2011 Murat Basbug et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. This study determined the effects of a single dose of bevacizumab, an antiangiogenic recombinant monoclonal antibody that specifically targets vascular endothelial growth factor (VEGF), on adhesion formation in the rat cecal abrasion model. Methodology. Thirty female Wistar albino rats (200–224 g) were divided into three groups. All rats underwent laparotomy at which time cecal wall abrasion and abdominal wall injuries were induced. Group I (control) underwent only the abrasion procedure; Groups II and III received saline or bevacizumab intraperitoneally, respectively, following the abrasion. The rats were killed on postoperative day 7, and the severity of adhesions was evaluated, together with histopathological fibrosis parameters and immunohistochemical staining to identify the VEGF receptor. Results. The mean adhesion severity score in Groups I–III was , , and , respectively; the score in Group III was significantly lower than that in Groups I ( ) and II ( ). In the histopathological evaluation, the mean fibrosis score in Group III was significantly lower that the scores in Groups I ( ) and II ( ). VEGF staining of the adhesion areas in Group III was significantly lower than that in Groups I ( ) and II ( ). Conclusion. Bevacizumab decreases adhesion formation following laparotomy in rats by blocking VEGF receptor occupancy.