The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease

Table 6

Immunohistochemical study of the Ki-67 expression in esophageal and gastric mucosa of GERD patients and HCs.

	GERD (𝑛=53)				Healthy controls (𝑛=15)
Grade of Ki-67 staining (n)	Esophagus		Stomach		Esophagus		Stomach
	BRI (+); (𝑛=25)	BRI (−); (𝑛=28)	BRI (+); (𝑛=25)	BRI (−); (𝑛=28)	BRI (+); (𝑛=2)	BRI (−); (𝑛=13)	BRI (+); (𝑛=2)	BRI (−); (𝑛=13)
0	—	—	—	—	—	—	—	—
1	17	17	11	17	1	9	1	9
2	3	3	4	—	1	3	—	3
3	2	5	3	5	—	1	—	1
4	3	2	4	3	—	—	1	—
5	—	1	3	3	—	—	—	—

According to the percentages of the stained epithelial cells, the immunohistological sections were scored and graded as follows: 0=absence of staining, 1=staining up to 5%, 2=staining>5%-10%, 3=staining>10%-25%,4=staining>25%-50%, and 5=staining>51%.