Colorectal Oncogenesis and Inflammation in a Rat Model Based on Chronic Inflammation due to Cycling DSS Treatments

Table 3

Liver injury. Liver status of different treatment groups of rats expressed as scoring values (degree of scoring: received value/maximum value) and incidence of phenomena/total number of animals. Percentages of the values are shown in parentheses. Incidence of translocation is mentioned in the following order: growth on Rogosa agar; Brain Heart Infusion agar (incubated anaerobically); Brain Heart Infusion agar (incubated aerobically). Fifteen isolates from the liver were subjected to 16S rDNA sequencing, and the similarity level was ≥99% for twelve of the samples, and 98.6% for one of the L. animalis (DSS group), 95.6% L. frumenti (antri) (DSS group), and 93.1% for one of the L. antri (DSS group). If the species is identified for more than one sample, the number is shown in parentheses.

Scoring values

Incidence

Groups

Degree of steatosis

Cell infiltration around CV within steatotic areas

Cellinfiltration elsewhere in the parenchyma

Stasis

Loss of parenchyma

Steatosis

Cell infiltration around CV within steatotic areas

Cell infiltration elsewhere in the parenchyma

Stasis

Loss of parenchyma

Translocation

Species designation of translocated bacteria

NC

10/21 (47.6%)

2/21; (9.5%)

0/21***

0/21

0/21

7/7

1/7

0/7***

0/7*

0/7

0/8; 1/8; 1/8

L. animalis

DSS

10/24; (41.7%)

2/24; (8.3%)

16/24; (66.7%)

8/21 (38.1%)

6/18; (33.3%)

8/8

1/8

8/8

4/7

3/6

3/8; 2/8; 4/8

L. animalis (2),; L. apodemi,; K. rhizophila,; L. frumenti; (L. antri),; L. antri (2),; L. gasseri,; M. luteus,; C. ramosum,; S. warneri (2)

AOM

4/18; (22.2%)

0/18

0/18***

0/18

0/18

2/6^#

0/6

0/6***

0/6*

0/6

0/7; 1/7; 2/7

K. rhizophila; C. perfringens

Scoring values: ***Denotes 𝑃<0.001 compared to the DSS group.
Incidence: ***Denotes 𝑃<0.001 and ^∗𝑃<0.05 compared to the DSS group, ^#𝑃<0.05 compared to the NC and DSS group Lactobacillus animalis (L. animalis), Lactobacillus apodemi (L. apodemi), Kocuria rhizophila (K. rhizophila), Lactobacillus frumenti (L. frumenti), Lactobacillus antri (L. antri), Lactobacillus gasseri (L. gasseri), Micrococcus luteus (M. luteus), and Clostridium ramosum (C. ramosum), Staphylococcus warneri (S. warneri), Clostridium perfringens (C. perfringens). Species shown in parentheses have the same sequence similarity as the previous species.