Gastroenterology Research and Practice / 2012 / Article / Fig 4

Research Article

Genetically Modified Lactococcus lactis for Delivery of Human Interleukin-10 to Dendritic Cells

Figure 4

(a) Depicted is the cell cycle progression of allogenic Th-cells cocultured with MF-derived effector Th-cells (white histograms, reference condition) and of allogenic Th-cells cocultured with effector Th-cells when no -IL-10 was added (no -IL-10), -IL-10 during DC-naïve T-cell ( -IL-10 DC-T) coculture or -IL-10 during effector T-cell and allogenic T-cell ( -IL-10 T-T) coculture (gray histograms). (b) Bar graphs represent the mean inhibition of fluorescence intensity of allogenic Th-cells for the indicated conditions in triplicate. The proliferation of allogenic Th-cells cocultured with effector Th-cells induced by MF-matured DC is regarded as 100% (reference condition). For transwell experiments, 2 × 106 PKH-labeled test cells were put in the upper compartment of a 24-well plate transwell system. Mean percentages with standard error of mean are given; **indicates using the -test for comparing percentages with the reference condition (α-IL-10 DC-T and transwell, resp.).

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