Figure 2: expression of zinc fingers and homeoboxes 2 (ZHX2), nuclear factor-YA (NF-YA), and alpha-fetal protein (AFP) mRNA in HepG2 cells after siRNA transfection. The target mRNA expression was detected by RT-PCR analysis. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as an internal control. The amounts of mRNA in the ZHX2 siRNA-treated cells were compared to those of control cells. After siRNA transfection, ZHX2 mRNA expression (389 bp) was decreased by 85%, NF-YA mRNA expression (117 bp) was reduced by 60%, and AFP mRNA expression (240 bp) was increased by 2.4-fold.