Expression Profiling of Proliferation and Apoptotic Markers along the Adenoma-Carcinoma Sequence in Familial Adenomatous Polyposis Patients

Table 1

Primary antibody sources, dilution, and antigen retrieval buffers and conditions.

Antibody	Source	Dilution	Antigen retrieval buffer	Microwaving time
Beta-catenin	LEICA NCL-B-CAT	1 : 100	Citrate	30 min
p53	LEICA NCL-p53-D07	1 : 100	Citrate	20 min
Bcl-2	DAKO; M0887	1 : 200	EDTA	30 min
Cyclin-D1	Thermo Shandon RM-9104-S	1 : 25	Citrate	40 min
Caspase-3	Cell Signalling CAT: 9664	1 : 800	EDTA	20 min
CD10	LEICA NCL-L-CD10-270	1 : 20	EDTA	30 min
Ki-67	LEICA NCL-L-KI67-MM1	1 : 100	Citrate	30 min