Research Article

LEPREL1 Expression in Human Hepatocellular Carcinoma and Its Suppressor Role on Cell Proliferation

Figure 2

The effect of LEPREL1 on cell growth and colony formation. ((a) and (b)) Exogenous LEPREL1 was expressed in Bel-7402 (a), HepG2 (b) cells transfected with the pcDNA3.1 vector. Parental cells with an empty vector were used as a control. A t-test was used to show significant differences between the two groups ( ). (c) To observe the effects of LEPREL1 on colony formation, pcDNA3.1-LEPREL1 was transfected into Bel-7402 and HepG2 cells. Twenty-four hours after the transfection, the cells were plated on the dishes and were cultured in G418 for two weeks. Representative photographs of the colony formation from different stable cell lines are shown in the left panels. The colony formation rate (%) is shown in the right panels (calculated by dividing the colony numbers by plated cells). The data (mean ± SEM) were obtained from three independent experiments ( ; ). The representative dishes showed the inhibitory effects of LEPREL1 on colony formation. The histogram shows that colony formation was significantly suppressed by LEPREL1 as compared with the empty vector control, where the numbers are the mean value of three independent experiments with SD.