Association of IS605 and cag-PAI of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan

Table 1

PCR primers used to detect CagA, CagE, CagM, CagT, orf10, orf13, TnpA, and TnpB in H. pylori isolates.

Genes	Primers	Nucleotide sequences (5′–3′)	Length of PCR products	Location in genome of reference^†
cagA	cagA-F	GATAACAGGCAAGCTTTTGAGG	349
	cagA-R	CTGCAAAAGATTGTTTGGCAGA		14994–15342
cagE	cagE-F	GTTACATCAAAAATAAAAGGAAGCG	735
	cagE-R	CAATAATTTTGAAGAGTTTCAAAGC		12342–13076
cagM	cagM-F	ACAAATACAAAAAAGAAAAAGAGGC	587
	cagM-R	ATTTTTCAACAAGTTAGAAAAAGCC		4815–9264
cagT	cagT-F	TCTAAAAAGATTACGCTCATAGGCG	490
	cagT-R	CTTTGGCTTGCATGTTCAAGTTGCC		1182–1671
orf10	orf10-F	AATAGTGCTTTCTTTAGGATTAGCG	658
	orf10-R	CCGATTTAATCCTTTCGCTTATGTG		8970–9627
orf13	orf13-F	CGTTCATGTTCCATACATCTTTGGC	617
	orf13-R	GATTTATAGCGATCTAAGAAACCGC		704–1320
tnpA	tnpA-F	ATCAGTCCAAAAAGTTTTTTCTTTCC	338
	tnpA-R	TAAGGGGGTATATTTCAACCAACCG		154–541
tnpB	tnpB-F	CGCTCTCCCTAAATTCAAAGAGGGC	578
	tnpB-R	AGCTAGGGAAAAATCTGTCTATGCC		954–1531

†GenBank accession number. AF282853.1.