Sodium Valproate Inhibits the Growth of Human Cholangiocarcinoma In Vitro and In Vivo
Figure 5
Apoptotic morphological changes in TFK-1 cells after treatment with sodium valproate. After 120 h, untreated control group (a) and 2 mM sodium valproate-treated TFK-1 cells (b) were loaded with Hoechst 33342/PI and then observed via fluorescence microscopy (100x magnification). Mixture of low blue with low pink fluorescence indicates live cells ((a), short arrow), while high blue fluorescence indicates apoptotic cells ((b), long arrow), and pink represents dead cells ((b), short arrow).
