Preventive Effect of TU-100 on a Type-2 Model of Colitis in Mice: Possible Involvement of Enhancing Adrenomedullin in Intestinal Epithelial Cells

Table 1

Screening for ingredients that enhance ADM production.

Test sample origin	Ingredient	Concentration (μmol/L)	Growth activity (optical density)	ADM production (pg/mL)
—	Control	—	1.419±0.045	85.3±4.9
Processed ginger	[6]-Gingerol	3	1.429±0.016	99.7±12.4
		30	1.400±0.025	93.0±6.5
	[6]-Shogaol	3	1.504±0.019	100.0±9.3
		30	1.363±0.023	246.3±5.0^∗
Ginseng radix	Ginsenoside Rb1	3	1.399±0.014	98.0±2.6
		30	1.366±0.047	90.7±9.2
	Ginsenoside Rg1	3	1.393±0.067	89.0±5.1
		30	1.375±0.040	95.7±3.8
	Ginsenoside Rd	3	1.379±0.045	88.3±5.4
		30	1.386±0.026	103.3±12.3
Japanese pepper	Xanthoxylin	3	1.429±0.030	89.3±7.4
		30	1.395±0.012	99.0±12.1
	Hydroxy α-sanshool	3	1.449±0.009	110.3±12.5
		30	1.557±0.013^∗	120.3±8.8^∗
		100	1.493±0.043	162.0±1.7^∗
Maltose syrup	Maltose	30	1.484±0.019	106.7±11.0
		300	1.467±0.031	106.7±11.7

IEC-6 cells were settled overnight and cultured for 1 d with or without the test sample at the indicated concentrations. ADM in the culture fluids was measured by EIA. Cell growth was measured using an XTT reduction assay kit. 𝑁=3. ^∗𝑃<0.05 versus control by Dunnett test.