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Gastroenterology Research and Practice
Volume 2014, Article ID 674875, 6 pages
http://dx.doi.org/10.1155/2014/674875
Research Article

The Effect of Peritoneal Air Exposure on Intestinal Mucosal Barrier

1Department of General Internal Medicine, Jiangsu Province Official Hospital, Nanjing 210024, China
2Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China
3Research Institute of General Surgery, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University, Nanjing 210002, China

Received 25 April 2014; Revised 17 July 2014; Accepted 4 August 2014; Published 20 August 2014

Academic Editor: Andrew Thillainayagam

Copyright © 2014 Jun Bao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Damage of the intestinal mucosa barrier may result in intestinal bacterial and endotoxin translocation, leading to local and systemic inflammation. The present study was designed to investigate whether peritoneal air exposure induces damage of intestinal mucosal barrier. Methods. Sprague-Dawley rats (weighing 210 to 230 g) were randomized into five groups (6/group): a control group, a sham group, and three exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. At 24 h after surgery, blood and terminal ileum were sampled. The serum D-lactate levels were determined using an ELISA kit. The intestinal permeability was determined by measuring the intestinal clearance of FITC-dextran (FD4). The histopathological changes in terminal ileum were also assessed. Results. Compared with the controls, peritoneal air exposure caused an increase in both serum D-lactate level and intestinal FD4 clearance, which were proportional to the length of peritoneal air exposure and correlated to Chiu’s scores, indices for intestinal mucosal injury. Edema and inflammatory cells were also observed in mucosa and submucosa of ileum in three exposure groups. Conclusions. Peritoneal air exposure could induce damage to the intestinal mucosal barrier, which is proportional to the time length of peritoneal air exposure.