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Gastroenterology Research and Practice
Volume 2015, Article ID 235017, 6 pages
http://dx.doi.org/10.1155/2015/235017
Research Article

Vascular Endothelial Injury and Apoptosis in Rats with Severe Acute Pancreatitis

1Division of Geriatric Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China
2Chinese Medicine Department, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China
3Cardiac Medicine Department, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, China

Received 12 October 2014; Accepted 5 January 2015

Academic Editor: Maria Eugenicos

Copyright © 2015 Ning Ge et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We explored mechanisms of vascular endothelial injury that lead to systemic multiple organ failure by detecting the soluble endothelial protein C receptor (sEPCR), von Willebrand factor (vWF), serum nitric oxide (NO), and tumor necrosis factor alpha (TNF-α) and Bcl-2 mRNA and Bax mRNA expression in a severe acute pancreatitis (SAP) rat model. Compared to controls, the levels of TNF-α, vWF, and sEPCR were significantly increased in the experimental group at 12 hours and 24 hours and the NO level was significantly decreased. After 12 hours, the aortic endothelial apoptosis index and Bax mRNA expression in aortic endothelial cells had increased in the experimental group, but Bcl-2 mRNA levels had decreased. All these changes appeared at both 12 h and 24 hours. The results indicated that vascular endothelial injury and apoptosis markers were elevated in SAP. Endothelial injury and increased apoptosis in the experimental group were related to the increased expression of TNF-α.