Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours

Figure 4

The role of MUPCDH 5′ DNA methylation for gene expression in colorectal cancer cell lines. (a) Comparison of MUPCDH expression and DNA methylation levels in four cell lines: HCT116, SW480, COLO205, and HT29; (b) results of the chromatin immunoprecipitation assay showing the level of RNA polymerase II binding as well as the levels of H3K4me3 and H3K27me3 at the MUPCDH promoter in four cell lines with diverse gene expression; (c) MUPCDH expression levels in colorectal cell lines cultured with various concentrations of DNA methyltransferase inhibitor, 5-aza-2′-deoxycytidine.

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