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Gastroenterology Research and Practice
Volume 2015, Article ID 327872, 9 pages
http://dx.doi.org/10.1155/2015/327872
Research Article

Herbal Substance, Acteoside, Alleviates Intestinal Mucositis in Mice

1Gastroenterology Unit, Women’s & Children’s Health Network, 72 King William Road, North Adelaide, SA 5006, Australia
2Discipline of Physiology, School of Medical Sciences, University of Adelaide, Adelaide, SA 5005, Australia
3Department of Pharmacognosy & Natural Products Chemistry, National and Kapodistrian University of Athens, 15771 Ilisia, Greece

Received 21 October 2014; Revised 4 December 2014; Accepted 4 December 2014

Academic Editor: Gianfranco D. Alpini

Copyright © 2015 Daniel Reinke et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated the role of acteoside in the amelioration of mucositis. C57BL/6 mice were gavaged daily with acteoside 600 μg for 5 d prior to induction of mucositis and throughout the experimental period. Mucositis was induced by methotrexate (MTX; 12.5 mg/kg; s.c.). Mice were culled on d 5 and d 11 after MTX. The duodenum, jejunum, and ileum were collected for myeloperoxidase (MPO) activity, metallothionein (MT) levels, and histology. Acteoside reduced histological severity scores by 75, 78, and 88% in the duodenum, jejunum, and ileum, respectively, compared to MTX-controls on d 5. Acteoside reduced crypt depth by 49, 51, and 33% and increased villus height by 19, 38, and 10% in the duodenum, jejunum, and ileum, respectively, compared to MTX-controls on d 5. Acteoside decreased MT by 50% compared to MTX-control mice on d 5. Acteoside decreased MPO by 60% and 30% in the duodenum and jejunum, respectively, compared to MTX-controls on d 5. Acteoside alleviated MTX-induced small intestinal mucositis possibly by preventing inflammation.