Table of Contents Author Guidelines Submit a Manuscript
Gastroenterology Research and Practice
Volume 2015 (2015), Article ID 750932, 6 pages
http://dx.doi.org/10.1155/2015/750932
Research Article

Expression of Helicobacter pylori hspA Gene in Lactococcus lactis NICE System and Experimental Study on Its Immunoreactivity

1Department of Pathogen Biology, Medical College, Henan University of Science and Technology, Luoyang 471003, China
2Department of Immunology, Medical College, Henan University of Science and Technology, Luoyang 471003, China
3Department of Epidemiology, Medical College, Henan University of Science and Technology, Luoyang 471003, China

Received 5 May 2014; Accepted 3 March 2015

Academic Editor: Spiros D. Ladas

Copyright © 2015 Xiao-Juan Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. P. Vyas and V. Sihorkar, “Exploring novel vaccines against Helicobacter pylori: protective and therapeutic immunization,” Journal of Clinical Pharmacy and Therapeutics, vol. 24, no. 4, pp. 259–272, 1999. View at Publisher · View at Google Scholar · View at Scopus
  2. V. De Francesco, A. Zullo, C. Hassan, F. Giorgio, R. Rosania, and E. Ierardi, “Mechanisms of Helicobacter pylori antibiotic resistance: an updated appraisal,” World Journal of Gastrointestinal Pathophysiology, vol. 2, no. 3, pp. 35–41, 2011. View at Publisher · View at Google Scholar
  3. A. Pietroiusti, M. Giuliano, A. Magrini, A. Bergamaschi, and A. Galante, “Cytotoxin-associated gene A strains of Helicobacter pylori represent a risk factor for the development of microalbuminuria in type 2 diabetes,” Diabetes Care, vol. 29, no. 6, pp. 1399–1401, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. K.-S. Wu, C.-C. Hsiao, H.-R. Yu, E.-Y. Huang, W.-L. Mai, and J.-M. Sheen, “Helicobacter pylori infection and childhood idiopathic thrombocytopenic purpura,” Acta Paediatrica Taiwanica, vol. 48, no. 5, pp. 263–266, 2007. View at Google Scholar · View at Scopus
  5. F. Franceschi, G. Niccoli, G. Ferrante et al., “CagA antigen of helicobacter pylori and coronary instability: insight from a clinico-pathological study and a meta-analysis of 4241 cases,” Atherosclerosis, vol. 202, no. 2, pp. 535–542, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. P. M. Norton, J. M. Wells, H. W. G. Brown, A. M. Macpherson, and R. W. F. Le Page, “Protection against tetanus toxin in mice nasally immunized with recombinant Lactococcus lactis expressing tetanus toxin fragment C,” Vaccine, vol. 15, no. 6-7, pp. 616–619, 1997. View at Publisher · View at Google Scholar · View at Scopus
  7. L. G. Bermúdez-Humarán, N. G. Cortes-Perez, F. Lefèvre et al., “A novel mucosal vaccine based on live Lactococci expressing E7 antigen and IL-12 induces systemic and mucosal immune responses and protects mice against human papillomavirus type 16-induced tumors,” The Journal of Immunology, vol. 175, no. 11, pp. 7297–7302, 2005. View at Publisher · View at Google Scholar · View at Scopus
  8. R. Ramasamy, S. Yasawardena, A. Zomer, G. Venema, J. Kok, and K. Leenhouts, “Immunogenicity of a malaria parasite antigen displayed by Lactococcus lactis in oral immunisations,” Vaccine, vol. 24, no. 18, pp. 3900–3908, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. S. Pavan, P. Hols, J. Delcour et al., “Adaptation of the nisin-controlled expression system in Lactobacillus plantarum: a tool to study in vivo biological effects,” Applied and Environmental Microbiology, vol. 66, no. 10, pp. 4427–4432, 2000. View at Publisher · View at Google Scholar · View at Scopus
  10. E. M. Bryan, T. Bae, M. Kleerebezem, and G. M. Dunny, “Improved vectors for nisin-controlled expression in gram-positive bacteria,” Plasmid, vol. 44, no. 2, pp. 183–190, 2000. View at Publisher · View at Google Scholar · View at Scopus
  11. S. J. Czinn, “Helicobacter pylori infection: detection, investigation, and management,” Journal of Pediatrics, vol. 146, no. 3, pp. S21–S26, 2005. View at Publisher · View at Google Scholar · View at Scopus
  12. G. Rossi, P. Ruggiero, S. Peppoloni et al., “Therapeutic vaccination against Helicobacter pylori in the beagle dog experimental model: safety, immunogenicity, and efficacy,” Infection and Immunity, vol. 72, no. 6, pp. 3252–3259, 2004. View at Publisher · View at Google Scholar · View at Scopus
  13. M. Pizza, M. M. Giuliani, M. R. Fontana et al., “Mucosal vaccines: non toxic derivatives of LT and CT as mucosal adjuvants,” Vaccine, vol. 19, no. 17–19, pp. 2534–2541, 2001. View at Publisher · View at Google Scholar · View at Scopus
  14. C. Xu, Z.-S. Li, Y.-D. Du et al., “Construction of a recombinant attenuated Salmonella typhimurium DNA vaccine carrying Helicobacter pylori hpaA,” World Journal of Gastroenterology, vol. 11, no. 1, pp. 114–117, 2005. View at Publisher · View at Google Scholar · View at Scopus
  15. I. Mierau and M. Kleerebezem, “10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis,” Applied Microbiology and Biotechnology, vol. 68, no. 6, pp. 705–717, 2005. View at Publisher · View at Google Scholar · View at Scopus
  16. T. Maischberger, I. Mierau, C. K. Peterbauer, J. Hugenholtz, and D. Haltrich, “High-level expression of Lactobocillusβ-galactosidases in Lactococcus lactis using the food-grade, nisin-controlled expression system NICE,” Journal of Agricultural and Food Chemistry, vol. 58, no. 4, pp. 2279–2287, 2010. View at Publisher · View at Google Scholar · View at Scopus
  17. A. Berlec and B. Štrukelj, “Large increase in brazzein expression achieved by changing the plasmid/strain combination of the NICE system in Lactococcus lactis,” Letters in Applied Microbiology, vol. 48, no. 6, pp. 750–755, 2009. View at Publisher · View at Google Scholar · View at Scopus
  18. I. Mierau, P. Leij, I. van Swam et al., “Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: the case of lysostaphin,” Microbial Cell Factories, vol. 4, article 15, 2005. View at Publisher · View at Google Scholar · View at Scopus
  19. S. J. Kim, J. Y. Lee, D. Y. Jun et al., “Oral administration of Lactococcus lactis expressing Helicobacter pylori Cag7-ct383 protein induces systemic anti-Cag7 immune response in mice,” FEMS Immunology and Medical Microbiology, vol. 57, no. 3, pp. 257–268, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. X. J. Zhang, G. C. Duan, R. G. Zhang, and Q. Fan, “Optimized expression of Helicobacter pylori ureB gene in the Lactococcus lactis nisin-controlled gene expression (NICE) system and experimental study of its immunoreactivity,” Current Microbiology, vol. 58, no. 4, pp. 308–314, 2009. View at Publisher · View at Google Scholar · View at Scopus