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Gastroenterology Research and Practice
Volume 2016 (2016), Article ID 2313850, 9 pages
Research Article

Members of the Cyr61/CTGF/NOV Protein Family: Emerging Players in Hepatic Progenitor Cell Activation and Intrahepatic Cholangiocarcinoma

1Department of Pathology and Laboratory Medicine, Rutgers, The State University of New Jersey, Newark, NJ 07103, USA
2Department of Pediatrics, University of Florida, Gainesville, FL 32610, USA

Received 21 July 2016; Revised 24 September 2016; Accepted 26 September 2016

Academic Editor: Andrea C. Gardini

Copyright © 2016 Qunfeng Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental materials and methods Ctgfp-GFP mice (eight-week old) were fed a 0.1% DDC-supplemented diet for 0 or 20 days before being sacrificed to determine the expression pattern of Ccn2/Ctgf promoter driven GFP in vivo. Liver slices were fixed in 4% paraformaldehyde. OCT embedding was performed for vimentin staining and paraffin embedding was for HNF4α and glutamine synthetase. In the immunofluorescent staining, 10% horse serum was used to block nonspecific signals. Primary antibodies included chicken anti-GFP, rabbit anti-HNF4α, mouse anti-glutamine synthetase (Abcam, Cambridge, MA), and chicken anti-vimentin (EnCor Biotechnology, Gainesville, FL). Alexa Fluor 488 conjugated donkey anti-goat and Alexa Fluor 594 conjugated donkey anti-rabbit secondary antibodies (Invitrogen, Carlsbad, CA) were used for detection. Images were taken under fluorescent microscope using DP80 color camera and cellSens software (Olympus, Pittsburgh, PA).

  1. Supplementary Material