Annexin A2 Coordinates STAT3 to Regulate the Invasion and Migration of Colorectal Cancer Cells In Vitro

Figure 2

Annexin A2 positively modulated the STAT3 phosphorylation in Caco-2 cells. (a) Relative mRNA level of Annexin A2 and STAT3 in the Caco-2 cells, which were transfected with pRC/CMV-Annexin A2-FLAG (Annexin A2 (+)), pcRC/CMV (Control (+)), siRNAi-Annexin A2 (Annexin A2 (KD)), or control siRNA (control (KD)) for 24 hours; (b) western blotting assay of Annexin A2 with or without Tyrosine 23 phosphorylation and STAT3 with or without Tyrosine 705 phosphorylation in the Caco-2 cell groups of Annexin A2 (+), control (+), Annexin A2 (KD), and control (KD); (c) relative protein levels of Annexin A2 and STAT3 without phosphorylation to β-actin in the abovementioned groups; (d) levels of phosphorylated forms to non-phosphorylated forms of Annexin A2 and STAT3 in the abovementioned groups; (e) coimmunoprecipitation analysis of the interaction between the phosphorylated Annexin A2 (Tyr 23) and the phosphorylated STAT3 (Tyr 705). Data was averaged for triple independent experiments, represented , represented , and ns represented no significance.

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