Cytokines as Early Markers of Colorectal Anastomotic Leakage: A Systematic Review and Meta-Analysis

Table 4

Specifying the methodology of cytokine level measurement of included studies. NS: not specified; ELISA: enzyme-linked immunosorbent assay.

Author	Year	Cytokines	Location	Centrifugation	Storage	Cytokine measuring	Producer
Bertram et al. [23]	2003	TNF, IL-6	Peritoneal	3000 rpm for 10 min at 4°C	−80°C	ELISA	Immulite, DPC Biermann GmbH, Bad Nauheim, Germany
Fouda et al. [21]	2011	TNF, IL-6, IL-10	Peritoneal	3000 rpm for 10 min at 4°C	20°C	ELISA	NS
Herwig et al. [24]	2002	TNF, IL-1β, IL-6	Peritoneal	2000 rpm for 10 min	−70°C	ELISA	Coulter-Immunotech Diagnostics, Hamburg, Germany
Matthiessen et al. [4, 19]	2007	TNF, IL-6, IL-10	Peritoneal	NS	NS	ELISA	DPC, Los Angeles, CA, USA
Yamamoto et al. [20]	2011	TNF, IL-1β, IL-6	Peritoneal	3000 rpm for 10 min	−80°C	ELISA	R&D system, Minneapolis, MN, USA
Ellebæk et al. [25]	2014	TNF, IL-1β, IL-6, IL-10	Systemic	3600 rpm for 10 min at 4°C	−80°C	ELISA	Bio-Rad Laboratories, Hercules, CA, USA
Reisinger et al. [26]	2014	IL-6	Systemic	3500 rpm for 15 min	−80°C	ELISA	NS