Treatment with a Monoclonal Anti-IL-12p40 Antibody Induces Substantial Gut Microbiota Changes in an Experimental Colitis Model
Table 1
Cytokine and chemokine levels in colon.
pg/100 mg colon
Mice group
Significance
Significance
SCID healthy control
12p40-mAb
rat-IgG2a
12p40-mAb versus rat-IgG2a
12p40-mAb versus SCID
CCL5
192 ± 72
229 ± 35
493 ± 62
<0.001
0.600
IL-2
182 ± 24
156 ± 19
95 ± 11
0.010
0.430
IL-5
69 ± 15
118 ± 37
31 ± 3
0.020
0.400
IL-9
1,489 ± 180
1,379 ± 194
683 ± 97
0.004
0.730
IL-10
164 ± 30
156 ± 22
91 ± 10
0.010
0.840
IL-13
932 ± 145
763 ± 122
392 ± 52
0.009
0.430
IP-10
926 ± 567
965 ± 180
3,446 ± 606
<0.001
0.930
KC
224 ± 44
167.5 ± 31
440 ± 61
<0.001
0.310
TNF-α
28 ± 5
24 ± 5
60 ± 7
<0.001
0.630
Cytokine and chemokine levels in colon were evaluated by Luminex assay and are depicted as pg per 100 mg colon tissue. Only three of the study groups were included in the analysis (12p40-mAb, rat-IgG2a, and SCID control mice).
