Albumin Binding Function: The Potential Earliest Indicator for Liver Function Damage

Figure 1

EPR spectrum of the 16-DS bound to albumin and with some unbounded 16-DS in serum. Spin labels bounded at albumin binding sites could be released free to the serum when albumin structure was impaired. The large circle part represents the low affinity binding sites to fatty acid in albumin at . The small circle part represents the free label in serum at . The area of the two circles should be deducted when calculating TBS. H and L, respectively, represent the number of 16-DS bounded to high and low affinity fatty acid binding sites. H/L reflects the fatty acid binding affinity of albumin.