Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-κB Pathway and TNF-α
Effects of SDC1 on CXCL-1 secretion and migration of neutrophils. Expression of CXCL-1 was assessed by ELISA. LPS was used to induce secretions of CXCL-1. (a) The level of secreted CXCL-1 was downregulated by SDC1 (). (b, c) Cell culture supernatants containing high concentrations of CXCL-1 were used to induce migration of rat neutrophils. CXCL-1 secretion promoted migration of neutrophils; this promoting effect was diminished when cells were transfected with mut-SDC1. The transmigration was measured with use of transwell inserts; crystal violet staining was used to observe the cell quantity change (, ).
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