Snapshot on a Pilot Metagenomic Study for the Appraisal of Gut Microbial Diversity in Mice, Cat, and Man

Figure 1

Impact of three different 16S-amplicon library preparation methods on the observed relative abundance of bacterial taxa. One unique sample of soil (known to have the highest microbial community complexity) was used to compare three distinct methods of molecular indexing such as MID (multiplex identifier) or Genoscreen-developed sample identifier multiplex (SIM). Five examples of final bacterial profiling are shown for each of the three methods used to illustrate the reproducibility of DNA extraction.

(a) Index and adaptors addition with one direct PCR

(b) Index addition with PCR and adaptors addition through ligation

(c) Genoscreen Protocol Metabiote®