Table of Contents Author Guidelines Submit a Manuscript
Gastroenterology Research and Practice
Volume 2017 (2017), Article ID 3089378, 9 pages
Research Article

Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis

1College of Animal Science and Technology, Key Laboratory of Animal Production and Production Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, China
2Departments of Medicine, Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, USA
3College of Animal Science and Technology, Key Lab of Preventive Veterinary Medicine in Jilin Province, Jilin Agricultural Science and Technology University, Jilin, Jilin 132101, China
4Institute of Virology, Wenzhou University, Wenzhou, Zhejiang 325027, China
5Schools of Pharmacy and Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China
6First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, China
7Institute of Military Veterinary Institute, Academy of Military Medical Science, Changchun, Jilin 130122, China
8Alcohol Research Center, University of Louisville School of Medicine, Louisville, KY 40202, USA
9Robley Rex VA Medical Center, Louisville, KY 40206, USA

Correspondence should be addressed to Weimin Luan and Wenke Feng

Received 11 January 2017; Revised 24 March 2017; Accepted 2 April 2017; Published 11 May 2017

Academic Editor: Cristiano Pagnini

Copyright © 2017 Liming Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aims. Nutrient deficiencies are common in patients with inflammatory bowel disease (IBD). Adipose tissue plays a critical role in regulating energy balance. Fibroblast growth factor 21 (FGF21) is an important endocrine metabolic regulator with emerging beneficial roles in lipid homeostasis. We investigated the impact of FGF21 in experimental colitis-induced epididymal white adipose tissue (eWAT) lipolysis. Methods. Mice were given 2.5% dextran sulfate sodium (DSS) ad libitum for 7 days to induce colitis. The role of FGF21 was investigated using antibody neutralization or knockout (KO) mice. Lipolysis index and adipose lipolytic enzymes were determined. In addition, 3T3-L1 cells were pretreated with IL-6, followed by recombinant human FGF21 (rhFGF21) treatment; lipolysis was assessed. Results. DSS markedly decreased eWAT/body weight ratio and increased serum concentrations of free fatty acid (FFA) and glycerol, indicating increased adipose tissue lipolysis. eWAT intracellular lipolytic enzyme expression/activation was significantly increased. These alterations were significantly attenuated in FGF21 KO mice and by circulating FGF21 neutralization. Moreover, DSS treatment markedly increased serum IL-6 and FGF21 levels. IL-6 pretreatment was necessary for the stimulatory effect of FGF21 on adipose lipolysis in 3T3-L1 cells. Conclusions. Our results demonstrate that experimental colitis induces eWAT lipolysis via an IL-6/FGF21-mediated signaling pathway.