The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas

Figure 2

The methylation status of CpG about BARF1and BHRF1 gene promoters (BSP). Each circle is one CpG site and filled circles are methylated CpG sites. (a, b) The methylation status of CpG about BARF1 and BHRF1 gene promoters before 5-aza treatment. There are 50 and 26 CpG sites in BARF1 and BHRF1 promoters, respectively. The methylation ratio of BARF1 before 5-aza in Raji, B95-8, GT39, and GT38 was 98.0%, 46.7%, 48.3%, and 95.3%, respectively, and that of BHRF1 was 83.3%, 32.7%, 16.0%, and 97.4%, respectively. (c, d) The methylation status of CpG about BARF1and BHRF1 gene promoters before and after 5-aza treatment. After 10 μmol/L 5-aza treatment, the methylation ratio of BARF1 in B95-8 and GT38 turned to 0 and 75.0% and that of BHRF1 turned to 0 and 52.6%, respectively.

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