The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas

Table 1

Sequences of primers for MSP, BSP, and real-time PCR in this study.

	Primers	Sequence (5′-3′)	Annealing temp (°C)	Product size (bp)
MSP	BARF1p-MF	GTTGGATTTAGTTATTTTGTCGTTC	73°C	142
	BARF1p-MR	TTATCATATAAACCTAAAACCCGTA
	BARF1p-UF	GTTGGATTTAGTTATTTTGTTGTTTG	70°C	142
	BARF1p-UR	TTATCATATAAACCTAAAACCCATA
	BHRF1p-MF	TTTGTATATTTGGTTAGTTGATCGA	68°C	218
	BHRF1p-MR	CGAAACGTAATACTTCCTAAAAACG
	BHRF1p-UF	TTTGTATATTTGGTTAGTTGATTGA	66°C	220
	BHRF1p-UR	CCCAAAACATAATACTTCCTAAAAACA
BSP	BARF1pBSP-F	GTTAGTTAGGTTGGTTAGGGTTTA	74°C	738
	BARF1pBSP-R	CTCCAATAAAAAATCAAAATAACTC
	BHRF1pBSP-F	AGAATTTAGAGGAAGGGAATTTTATAGT	73°C	714
	BHRF1pBSP-R	ACAACCAAAACAAAAATAAAAAAAA
qRT-PCR	BARF1-F	AGCCTCTCTGTTGCTGTTGA	59°C	130
	BARF1-R	AGTGCGTTTATTGCGACAAG
	BHRF1-F	GTACCCTGCATCCTGTGTTG	59°C	72
	BHRF1-R	CTACAGTGTCCTCTGGCGAA
	GAPDH-F	CTCAGACACCATGGGGAAGGTGA	58°C	450
	GAPDH-R	ATGATCTTGAGGCTGTTGTCATA