The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas

Table 3

The mRNA expression of BARF1 and BHRF1 in cell lines before and after 5-Aza-CdR treatment.

Cell line	Treatment	mRNA relative expression to GAPDH(2^−ΔCt)
		BARF1	BHRF1
Raji	Untreated	2.5 × 10^−7	0.04
	10 μmol/L 5-aza	1.6 × 10^−6	0.09
	Ratio (after/before)	6.4	2.3
B95-8	Untreated	0.30	0.23
	10 μmol/L 5-aza	0.64	0.78
	Ratio (after/before)	2.1	3.4
GT39	Untreated	6.4 × 10^−4	2.4 × 10^−4
	10 μmol/L 5-aza	1.6 × 10^−3	4.0 × 10^−3
	Ratio (after/before)	2.5	16.7
GT38	Untreated	6.7 × 10^−3	0.04
	10 μmol/L 5-aza	0.49	0.26
	Ratio (after/before)	73.1	6.5
SNU719	Untreated	1.8 × 10^−3	0.02
	10 μmol/L 5-aza	10.4 × 10^−3	0.022
	Ratio (after/before)	5.8	1.1