Gastroenterology Research and Practice / 2017 / Article / Tab 3

Research Article

The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas

Table 3

The mRNA expression of BARF1 and BHRF1 in cell lines before and after 5-Aza-CdR treatment.

Cell lineTreatmentmRNA relative expression to GAPDH(2−ΔCt)
BARF1BHRF1

RajiUntreated2.5 × 10−70.04
10 μmol/L 5-aza1.6 × 10−60.09
Ratio (after/before)6.42.3
B95-8Untreated0.300.23
10 μmol/L 5-aza0.640.78
Ratio (after/before)2.13.4
GT39Untreated6.4 × 10−42.4 × 10−4
10 μmol/L 5-aza1.6 × 10−34.0 × 10−3
Ratio (after/before)2.516.7
GT38Untreated6.7 × 10−30.04
10 μmol/L 5-aza0.490.26
Ratio (after/before)73.16.5
SNU719Untreated1.8 × 10−30.02
10 μmol/L 5-aza10.4 × 10−30.022
Ratio (after/before)5.81.1