Quercetin Pretreatment Attenuates Hepatic Ischemia Reperfusion-Induced Apoptosis and Autophagy by Inhibiting ERK/NF-κB Pathway

Figure 6

Effects of QE on the regulation of the ERK/NF-κB pathway in mice on hepatic ischemia-reperfusion injury. Notes: (a) the mRNA level of NF-κB was detected by qRT-PCR (, for sham versus IR, ^# for QE100 + IR versus IR, and ⁺ for QE200 + IR versus IR). (b) The expression of ERK, p-ERK, and NF-κB was determined by Western blotting. (c) Immunohistochemistry staining (×200) showed the expression of p-ERK and NF-κB protein in liver tissues at 8 h. The ratio of positive area to total area was counted with Image-Pro Plus (v 6.0) (, for sham versus IR, ^# for QE100 + IR versus IR, and ⁺ for QE200 + IR versus IR).

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