Water Carcinogenicity and Prevalence of HPV Infection in Esophageal Cancer Patients in Huaihe River Basin, China

Table 1

The general primer sets for HPV DNA detection.

Primer name	Sequences
GP5+	5-TTTGTTACTGTGGTAGATACTAC-3
GP6+	5-GAAAAATAAACTGTAAATCATATTC-3
My09	5-CGTCCMARRGGAWACTGATC-3
My11	5-GCMCAGGGWCATAAYAATGG-3
HPV16F	5-CAACAAGACATACATCGACCG-3
HPV16R	5-TGGAACAACATTAGAACAGCAA-3
HPV18F	5-GCGCTTTGAGGATCCAACAC-3
HPV18R	5-ATTCAACGGTTTCTGGCAC-3

21 HPV GenoArray diagnostic kit (Hybribio Limited©) was also used for rapid and accurate HPV genotyping. It can identify 21 human papillomaviruses including 15 high-risk HPV types (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, and 68) and 6 low-risk HPV types.