EI24 Suppresses Tumorigenesis in Pancreatic Cancer via Regulating c-Myc

Figure 4

EI24 attenuates cell proliferation by inducing autophagy to regulate the Beclin-1/p62/c-Myc signaling pathway. (a) Expression level of caspase-3 and cleaved caspase-3 was detected by Western blot. (b) Western blot analysis shows cyclinD1, p21, and c-Myc protein levels in cancer cells. (c, d) Immunofluorescence analysis of Beclin-1 and p62 expression in xenograft tumor tissues. EI24 increased merged fluorescence image of Beclin-1 (red, Cy3 stained) with the nucleus (blue, DAPI staining) was observed, accompanied with a decrease of p62. Photos were telegraphed with 400x magnification. Scale bar represents 50 μm. (e) Expression levels of EI24-induced autophagy-related protein LC3B and Beclin-1 were detected by Western blot. , , and NS , compared with controls.

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