Fucosterol Protects against Concanavalin A-Induced Acute Liver Injury: Focus on P38 MAPK/NF-κB Pathway Activity

Figure 4

Effects of fucosterol on inflammatory factors in the acute liver injury model. (a) Serum levels of TNF-α, IL-6, and IL-1β 2, 8, and 24 h after ConA injection. (b) TNF-α, IL-6, IL-1β, and PPARγ mRNA levels were evaluated by quantitative real-time PCR. (c) Western blot and analysis of TNF-α, IL-6, IL-1β, PPARγ, NF-κB p65, and p-NF-κB p65 protein expression. (d) Immunohistochemistry was used to detect PPARγ and p-NF-κB p65 expression at 8 h. Original magnification: 200x. Data are expressed as mean ± SD (, for ConA versus control, for ConA + fucosterol (25 mg/kg) versus ConA, for ConA + fucosterol (50 mg/kg) versus ConA + fucosterol (25 mg/kg), and for ConA + fucosterol (100 mg/kg) versus ConA + fucosterol (50 mg/kg)).

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